SEPARATION OF NUCLEI REPRESENTING DIFFERENT PHASES OF THE GROWTH CYCLE FROM UNSYNCHRONIZED MAMMALIAN CELL CULTURES

Nuclei have been isolated from unsynchronized cultures of Chinese hamster fibroblasts after varying intervals of growth following the incorporation of thymidine (-3)H for 20 min. These nuclei were fractionated by unit gravity sedimentation in a stabilizing density gradient of sucrose, and fractions...

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Detalles Bibliográficos
Autores principales: McBride, O. Wesley, Peterson, Elbert A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108399/
https://www.ncbi.nlm.nih.gov/pubmed/5513550

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108399/
https://www.ncbi.nlm.nih.gov/pubmed/5513550

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