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CYTOCHEMICAL LOCALIZATION OF 5'-NUCLEOTIDASE IN SUBCELLULAR FRACTIONS ISOLATED FROM RAT LIVER : I. The Origin of 5'-Nucleotidase Activity in Microsomes
A procedure has been developed for the cytochemical localization of 5'-nucleotidase in isolated, unfixed, rat liver microsomes. Membranes were incubated with adenosine 5'-phosphate (5'-AMP) and Pb(NO(3))(2) and then isolated on sucrose density gradients: all the phosphate released was...
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| Formato: | Texto |
| Lenguaje: | English |
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The Rockefeller University Press
1972
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108658/ https://www.ncbi.nlm.nih.gov/pubmed/5009518 |