FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS

The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed...

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Detalles Bibliográficos
Autores principales: Plattner, Helmut, Fischer, Walter M., Schmitt, Werner W., Bachmann, Luis
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108701/
https://www.ncbi.nlm.nih.gov/pubmed/4622424

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108701/
https://www.ncbi.nlm.nih.gov/pubmed/4622424

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