FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS

The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed...

Descripción completa

Detalles Bibliográficos
Autores principales: Plattner, Helmut, Fischer, Walter M., Schmitt, Werner W., Bachmann, Luis
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108701/
https://www.ncbi.nlm.nih.gov/pubmed/4622424
_version_ 1782139084543623168
author Plattner, Helmut
Fischer, Walter M.
Schmitt, Werner W.
Bachmann, Luis
author_facet Plattner, Helmut
Fischer, Walter M.
Schmitt, Werner W.
Bachmann, Luis
author_sort Plattner, Helmut
collection PubMed
description The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed. In many instances the resultant morphology differed significantly from that obtained from glycerol-treated, freeze-etched cells. Furthermore, viability studies of spray-frozen Chlorella compared favorably with cells frozen by other methods.
format Text
id pubmed-2108701
institution National Center for Biotechnology Information
language English
publishDate 1972
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21087012008-05-01 FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS Plattner, Helmut Fischer, Walter M. Schmitt, Werner W. Bachmann, Luis J Cell Biol Article The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed. In many instances the resultant morphology differed significantly from that obtained from glycerol-treated, freeze-etched cells. Furthermore, viability studies of spray-frozen Chlorella compared favorably with cells frozen by other methods. The Rockefeller University Press 1972-04-01 /pmc/articles/PMC2108701/ /pubmed/4622424 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Plattner, Helmut
Fischer, Walter M.
Schmitt, Werner W.
Bachmann, Luis
FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS
title FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS
title_full FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS
title_fullStr FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS
title_full_unstemmed FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS
title_short FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS
title_sort freeze etching of cells without cryoprotectants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108701/
https://www.ncbi.nlm.nih.gov/pubmed/4622424
work_keys_str_mv AT plattnerhelmut freezeetchingofcellswithoutcryoprotectants
AT fischerwalterm freezeetchingofcellswithoutcryoprotectants
AT schmittwernerw freezeetchingofcellswithoutcryoprotectants
AT bachmannluis freezeetchingofcellswithoutcryoprotectants

Ejemplares similares