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FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS
The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1972
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108701/ https://www.ncbi.nlm.nih.gov/pubmed/4622424 |