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FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS : Isolation of Nuclear Membranes

0.3–0.5 M MgCl(2) was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55–60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sect...

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Detalles Bibliográficos
Autores principales: Monneron, Ariane, Blobel, Günter, Palade, George E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108744/
https://www.ncbi.nlm.nih.gov/pubmed/4120072