FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS : Isolation of Nuclear Membranes

0.3–0.5 M MgCl(2) was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55–60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sect...

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Detalles Bibliográficos
Autores principales: Monneron, Ariane, Blobel, Günter, Palade, George E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108744/
https://www.ncbi.nlm.nih.gov/pubmed/4120072
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author Monneron, Ariane
Blobel, Günter
Palade, George E.
author_facet Monneron, Ariane
Blobel, Günter
Palade, George E.
author_sort Monneron, Ariane
collection PubMed
description 0.3–0.5 M MgCl(2) was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55–60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sectioning, freeze etching, and negative staining procedures.
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spelling pubmed-21087442008-05-01 FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS : Isolation of Nuclear Membranes Monneron, Ariane Blobel, Günter Palade, George E. J Cell Biol Article 0.3–0.5 M MgCl(2) was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55–60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sectioning, freeze etching, and negative staining procedures. The Rockefeller University Press 1972-10-01 /pmc/articles/PMC2108744/ /pubmed/4120072 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Monneron, Ariane
Blobel, Günter
Palade, George E.
FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS : Isolation of Nuclear Membranes
title FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS : Isolation of Nuclear Membranes
title_full FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS : Isolation of Nuclear Membranes
title_fullStr FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS : Isolation of Nuclear Membranes
title_full_unstemmed FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS : Isolation of Nuclear Membranes
title_short FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS : Isolation of Nuclear Membranes
title_sort fractionation of the nucleus by divalent cations : isolation of nuclear membranes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108744/
https://www.ncbi.nlm.nih.gov/pubmed/4120072
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AT blobelgunter fractionationofthenucleusbydivalentcationsisolationofnuclearmembranes
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