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HORMONE SECRETION BY CELLS DISSOCIATED FROM RAT ANTERIOR PITUITARIES
A new procedure has been developed for dissociating anterior pituitary tissue and producing a viable suspension of single cells. The procedure involves incubation of small tissue blocks in 1 mg/ml trypsin (15 min), followed by incubation in 8 µg/ml neuraminidase and 1 mM EDTA (15 min), followed by m...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1973
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109084/ https://www.ncbi.nlm.nih.gov/pubmed/4375681 |