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Distribution of fluorescently labeled actin in living sea urchin eggs during early development
Rabbit skeletal muscle actin was labeled with 5- iodoacetamidofluorescein (5-IAF) and purified by gel filtration, ion- exchange chromatography, and polymerization-depolymerization. The resultant fluorescent conjugates retained full biochemical activities. The labeled actin was incorporated into unfe...
Formato: | Texto |
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Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1979
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110395/ https://www.ncbi.nlm.nih.gov/pubmed/457779 |