Identification and characterization of a membrane component essential for the translocation of nascent proteins across the membrane of the endoplasmic reticulum
When rough microsomes are subjected to limited proteolysis and high salt, a soluble fraction can be separated from the membrane. Neither fraction alone is capable of vectorially translocating nascent peptides. When the soluble extract is recombined with the residual membrane fraction, translocating...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1980
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110739/ https://www.ncbi.nlm.nih.gov/pubmed/7430254 |
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collection | PubMed |
description | When rough microsomes are subjected to limited proteolysis and high salt, a soluble fraction can be separated from the membrane. Neither fraction alone is capable of vectorially translocating nascent peptides. When the soluble extract is recombined with the residual membrane fraction, translocating activity is restored. Standard biochemical techniques were used to identify and characterize the active component derived by treating rough microsomes with elastase and high salt. The active factor is a peptide fragment with an apparent molecular weight of 60,000. It represents the cytoplasmic domain of a larger membrane protein. The fragment is basic and has at least one accessible sulfhydryl group. These characteristics facilitated its purification and identification as a membrane component required for translocation of nascent peptides across microsomal membranes. |
format | Text |
id | pubmed-2110739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1980 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21107392008-05-01 Identification and characterization of a membrane component essential for the translocation of nascent proteins across the membrane of the endoplasmic reticulum J Cell Biol Articles When rough microsomes are subjected to limited proteolysis and high salt, a soluble fraction can be separated from the membrane. Neither fraction alone is capable of vectorially translocating nascent peptides. When the soluble extract is recombined with the residual membrane fraction, translocating activity is restored. Standard biochemical techniques were used to identify and characterize the active component derived by treating rough microsomes with elastase and high salt. The active factor is a peptide fragment with an apparent molecular weight of 60,000. It represents the cytoplasmic domain of a larger membrane protein. The fragment is basic and has at least one accessible sulfhydryl group. These characteristics facilitated its purification and identification as a membrane component required for translocation of nascent peptides across microsomal membranes. The Rockefeller University Press 1980-11-01 /pmc/articles/PMC2110739/ /pubmed/7430254 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Identification and characterization of a membrane component essential for the translocation of nascent proteins across the membrane of the endoplasmic reticulum |
title | Identification and characterization of a membrane component essential for the translocation of nascent proteins across the membrane of the endoplasmic reticulum |
title_full | Identification and characterization of a membrane component essential for the translocation of nascent proteins across the membrane of the endoplasmic reticulum |
title_fullStr | Identification and characterization of a membrane component essential for the translocation of nascent proteins across the membrane of the endoplasmic reticulum |
title_full_unstemmed | Identification and characterization of a membrane component essential for the translocation of nascent proteins across the membrane of the endoplasmic reticulum |
title_short | Identification and characterization of a membrane component essential for the translocation of nascent proteins across the membrane of the endoplasmic reticulum |
title_sort | identification and characterization of a membrane component essential for the translocation of nascent proteins across the membrane of the endoplasmic reticulum |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110739/ https://www.ncbi.nlm.nih.gov/pubmed/7430254 |