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A permeabilized cell model for studying cytokinesis using mammalian tissue culture cells
PtK1 cells lysed late in cell division in a medium containing the nonionic detergent Brij 58 and polyethylene glycol with continue to undergo cleavage after lysis. Maintenance of cleavage after lysis is dependent on the composition of the lysis medium; the pH must be around neutrality, MgATP must be...
Formato: | Texto |
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Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1980
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110752/ https://www.ncbi.nlm.nih.gov/pubmed/7000792 |