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Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus)
We isolated calcium-labile mitotic spindles from eggs of the sea urchin Lytechinus variegatus, using a low ionic strength, EGTA lysis buffer that contined 5.0 mM EGTA, 0.5 mM MgCl2, 10-50 mM PIPES, pH 6.8, with 1% Nonidet P-40 (detergent) and 20-25% glycerol. Isolated spindles were stored in EGTA bu...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1980
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111485/ https://www.ncbi.nlm.nih.gov/pubmed/7190569 |