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A simple method for freeze-fracture of monolayer cultures
A simple method is described for the freeze-fracture in situ of monolayer cultures grown on gold carriers coated with a thin layer of silicon monoxide. Preliminary observations on 3T3 mouse embryo fibroblasts indicate that this technique exposes large areas of cell membrane, making it possible to de...
Formato: | Texto |
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Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1975
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111649/ https://www.ncbi.nlm.nih.gov/pubmed/172516 |