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A simple method for freeze-fracture of monolayer cultures
A simple method is described for the freeze-fracture in situ of monolayer cultures grown on gold carriers coated with a thin layer of silicon monoxide. Preliminary observations on 3T3 mouse embryo fibroblasts indicate that this technique exposes large areas of cell membrane, making it possible to de...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1975
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111649/ https://www.ncbi.nlm.nih.gov/pubmed/172516 |
| _version_ | 1782139772553134080 |
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| collection | PubMed |
| description | A simple method is described for the freeze-fracture in situ of monolayer cultures grown on gold carriers coated with a thin layer of silicon monoxide. Preliminary observations on 3T3 mouse embryo fibroblasts indicate that this technique exposes large areas of cell membrane, making it possible to determine how areas of membrane specialization are related to the cell as a whole and to regions of cellular interaction. 3T3 cells cultured on silicon monoxide show no modification of growth properties compared to cells growing on Falcon plastic, and other cell lines also appear to grow well on this substrate. |
| format | Text |
| id | pubmed-2111649 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 1975 |
| publisher | The Rockefeller University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-21116492008-05-01 A simple method for freeze-fracture of monolayer cultures J Cell Biol Articles A simple method is described for the freeze-fracture in situ of monolayer cultures grown on gold carriers coated with a thin layer of silicon monoxide. Preliminary observations on 3T3 mouse embryo fibroblasts indicate that this technique exposes large areas of cell membrane, making it possible to determine how areas of membrane specialization are related to the cell as a whole and to regions of cellular interaction. 3T3 cells cultured on silicon monoxide show no modification of growth properties compared to cells growing on Falcon plastic, and other cell lines also appear to grow well on this substrate. The Rockefeller University Press 1975-12-01 /pmc/articles/PMC2111649/ /pubmed/172516 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| spellingShingle | Articles A simple method for freeze-fracture of monolayer cultures |
| title | A simple method for freeze-fracture of monolayer cultures |
| title_full | A simple method for freeze-fracture of monolayer cultures |
| title_fullStr | A simple method for freeze-fracture of monolayer cultures |
| title_full_unstemmed | A simple method for freeze-fracture of monolayer cultures |
| title_short | A simple method for freeze-fracture of monolayer cultures |
| title_sort | simple method for freeze-fracture of monolayer cultures |
| topic | Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111649/ https://www.ncbi.nlm.nih.gov/pubmed/172516 |