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Detection of actin-binding proteins in human platelets by 125I-actin overlay of polyacrylamide gels

Actin-binding proteins have been identified in human platelets with a gel-overlay technique that uses 125I-G-actin. Platelet proteins were separated on SDS polyacrylamide gels using the buffer system of Laemmli (1970, Nature [Lond.] 227:680-685). The proteins were fixed in the gels with methanol-ace...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1981
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111894/
https://www.ncbi.nlm.nih.gov/pubmed/6793603