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Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center

Purified keratin, solubilized in 8 M of urea, was added to Triton X-100- extracted PtK1 cells in 5 mM PIPES buffer. The buffer conditions induced assembly of keratin filaments which appear to associate with nuclei of extracted cells. These keratin fibers extend beyond the original margin of the cell...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112089/
https://www.ncbi.nlm.nih.gov/pubmed/6174532