Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center

Purified keratin, solubilized in 8 M of urea, was added to Triton X-100- extracted PtK1 cells in 5 mM PIPES buffer. The buffer conditions induced assembly of keratin filaments which appear to associate with nuclei of extracted cells. These keratin fibers extend beyond the original margin of the cell...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112089/
https://www.ncbi.nlm.nih.gov/pubmed/6174532
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collection PubMed
description Purified keratin, solubilized in 8 M of urea, was added to Triton X-100- extracted PtK1 cells in 5 mM PIPES buffer. The buffer conditions induced assembly of keratin filaments which appear to associate with nuclei of extracted cells. These keratin fibers extend beyond the original margin of the cells and frequently form bridges between adjacent cells. Electron microscopy shows that keratin filaments associate closely with the surface of the nucleus. We suggest that the site of association between keratin and the nucleus may represent an intermediate filament organizing center.
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spelling pubmed-21120892008-05-01 Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center J Cell Biol Articles Purified keratin, solubilized in 8 M of urea, was added to Triton X-100- extracted PtK1 cells in 5 mM PIPES buffer. The buffer conditions induced assembly of keratin filaments which appear to associate with nuclei of extracted cells. These keratin fibers extend beyond the original margin of the cells and frequently form bridges between adjacent cells. Electron microscopy shows that keratin filaments associate closely with the surface of the nucleus. We suggest that the site of association between keratin and the nucleus may represent an intermediate filament organizing center. The Rockefeller University Press 1982-02-01 /pmc/articles/PMC2112089/ /pubmed/6174532 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center
title Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center
title_full Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center
title_fullStr Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center
title_full_unstemmed Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center
title_short Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center
title_sort assembly of keratin onto ptk1 cytoskeletons: evidence for an intermediate filament organizing center
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112089/
https://www.ncbi.nlm.nih.gov/pubmed/6174532

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