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Direct cytochemical localization of catalytic subunits dissociated from cAMP-dependent protein kinase in Reuber H-35 hepatoma cells. I. Development and validation of fluorescinated inhibitor
A specific and sensitive procedure has been developed that reliably localizes intracellular sites of free catalytic unit (C) dissociated from cAMP-dependent protein kinase. The method is based on a FITC conjugate (F:PKI) of affinity column-purified heat-stable protein inhibitor (PKI) of free C. The...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1982
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112138/ https://www.ncbi.nlm.nih.gov/pubmed/6288732 |