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Production of monoclonal antibodies against calmodulin by in vitro immunization of spleen cells
Monoclonal antibodies against the highly conserved ubiquitous calcium- binding protein, calmodulin (CaM), were produced by immunization of mouse primary spleen cell cultures. Dissociated spleen cells were cultured for 5 d in the presence of mixed thymocyte culture conditioned media (TCM) and purifie...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1983
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112333/ https://www.ncbi.nlm.nih.gov/pubmed/6833395 |