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Studies on conformation of F-actin in muscle fibers in the relaxed state, rigor, and during contraction using fluorescent phalloidin
F-actin in a glycerinated muscle fiber was specifically labeled with fluorescent phalloidin-(fluorescein isothiocyanate) FITC complex at 1:1 molar ratio. Binding of phalloidin-FITC to F-actin affected neither contraction of the fiber nor its regulation by Ca2+. Comparison of polarized fluorescence f...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1983
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112731/ https://www.ncbi.nlm.nih.gov/pubmed/6417144 |