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Direct measurement of actin polymerization rate constants by electron microscopy of actin filaments nucleated by isolated microvillus cores
We used actin filament bundles isolated from intestinal brush-border microvilli to nucleate the polymerization of pure muscle actin monomers into filaments. Growth rates were determined by electron microscopy by measuring the change in the length of the filaments as a function of time. The linear de...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1981
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112767/ https://www.ncbi.nlm.nih.gov/pubmed/6894301 |