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Thyrotropin-releasing hormone-induced changes in intracellular [Ca2+] measured by microspectrofluorometry on individual quin2-loaded cells
We have developed an accurate and practical method for measuring intracellular Ca2+ concentration [( Ca2+]i) in single cells in monolayer culture using the fluorescent Ca2+-binding dye quin2. Quin2 was loaded into cells as a membrane-permeant ester which is hydrolyzed in the cytoplasm to the imperme...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1984
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113418/ https://www.ncbi.nlm.nih.gov/pubmed/6432803 |