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Solubilization of proteins from bovine brain coated vesicles by protein perturbants and Triton X-100
To identify integral and peripheral membrane proteins, highly purified coated vesicles from bovine brain were exposed to solutions of various pH, ionic strength, and concentrations of the nonionic detergent Triton X-100. At pH 10.0 or above most major proteins were liberated, but four minor polypept...
Formato: | Texto |
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Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1985
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113649/ https://www.ncbi.nlm.nih.gov/pubmed/2861205 |