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Digital imaging fluorescence microscopy: spatial heterogeneity of photobleaching rate constants in individual cells
Photobleaching and related photochemical processes are recognized experimental barriers to quantification of fluorescence by microscopy. We have measured the kinetics of photobleaching of fluorophores in living and fixed cells and in microemulsions, and have demonstrated the spatial variability of t...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1985
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113759/ https://www.ncbi.nlm.nih.gov/pubmed/3920227 |