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In vitro reconstitution of exocytosis from plasma membrane and isolated secretory vesicles
We describe the reconstitution of exocytotic function through recombination of purified cortical secretory vesicles (CVs) and plasma membrane from sea urchin eggs. CVs were dislodged from a cell surface complex preparation by gentle homogenization in an isotonic dissociation buffer, and purified by...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1985
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114008/ https://www.ncbi.nlm.nih.gov/pubmed/2999160 |