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In situ hybridization at the electron microscope level: localization of transcripts on ultrathin sections of Lowicryl K4M-embedded tissue using biotinylated probes and protein A-gold complexes
A technique has been developed for localizing hybrids formed in situ on semi-thin and ultrathin sections of Lowicryl K4M-embedded tissue. Biotinylated dUTP (Bio-11-dUTP and/or Bio-16-dUTP) was incorporated into mitochondrial rDNA and small nuclear U1 probes by nick- translation. The probes were hybr...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1986
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114214/ https://www.ncbi.nlm.nih.gov/pubmed/3084498 |