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Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis
We describe an in vitro system with all components derived from the yeast Saccharomyces cerevisiae that can translocate a yeast secretory protein across microsomal membranes. In vitro transcribed prepro-alpha- factor mRNA served to program a membrane-depleted yeast translation system. Translocation...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1986
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114218/ https://www.ncbi.nlm.nih.gov/pubmed/3517001 |