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Microinjection of ubiquitin: intracellular distribution and metabolism in HeLa cells maintained under normal physiological conditions
Radioiodinated ubiquitin was introduced into HeLa cells by erythrocyte- mediated microinjection. Subsequent electrophoretic analyses revealed that the injected ubiquitin molecules were rapidly conjugated to HeLa proteins. At equilibrium, 10% of the injected ubiquitin was conjugated to histones and 4...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1987
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114525/ https://www.ncbi.nlm.nih.gov/pubmed/3029141 |