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In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins
An in vitro system was developed that provides a quick microscopic assay for nuclear transport. The assay uses an extract of Xenopus eggs, normal or synthetic nuclei, and a fluorescently labeled nuclear protein, nucleoplasmin. This in vitro system accurately mimics in vivo nuclear transport, both in...
Formato: | Texto |
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Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1986
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114618/ https://www.ncbi.nlm.nih.gov/pubmed/3097026 |