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Resting chondrocytes in culture survive without growth factors, but are sensitive to toxic oxygen metabolites
Chondrocytes in dense suspension culture in agarose survive in serum- free DME because they secrete low molecular mass compounds supporting their own viability. This activity can be replaced by pyruvate, or sulfhydryl compounds, e.g., cysteine or dithioerythritol. Catalase, an enzyme decomposing H2O...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1990
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2116165/ https://www.ncbi.nlm.nih.gov/pubmed/2365733 |