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Mitotic chromatin condensation in vitro using somatic cell extracts and nuclei with variable levels of endogenous topoisomerase II
We report the development of a new method for producing mitotic extracts from tissue culture cells. These extracts reproducibly promote the condensation of chromatin in vitro when incubated with purified interphase nuclei. This condensation reaction is not species specific, since nuclei from chicken...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1990
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2116389/ https://www.ncbi.nlm.nih.gov/pubmed/2176652 |