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In vitro reconstitution of intercompartmental protein transport to the yeast vacuole
Toward a detailed understanding of protein sorting in the late secretory pathway, we have reconstituted intercompartmental transfer and proteolytic maturation of a yeast vacuolar protease, carboxypeptidase Y (CPY). This in vitro reconstitution uses permeabilized yeast spheroplasts that are first rad...
Formato: | Texto |
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Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1990
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2116412/ https://www.ncbi.nlm.nih.gov/pubmed/2269659 |