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Development of an open-tubular trypsin reactor for on-line digestion of proteins
A study was initiated to construct a micro-reactor for protein digestion based on trypsin-coated fused-silica capillaries. Initially, surface plasmon resonance was used both for optimization of the surface chemistry applied in the preparation and for monitoring the amount of enzyme that was immobili...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2117336/ https://www.ncbi.nlm.nih.gov/pubmed/17899035 http://dx.doi.org/10.1007/s00216-007-1584-5 |