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Facilitation of Th1-mediated immune response by prostaglandin E receptor EP1

Prostaglandin E(2) (PGE(2)) exerts its actions via four subtypes of the PGE receptor, EP1–4. We show that mice deficient in EP1 exhibited significantly attenuated Th1 response in contact hypersensitivity induced by dinitrofluorobenzene (DNFB). This phenotype was recapitulated in wild-type mice by ad...

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Autores principales: Nagamachi, Miyako, Sakata, Daiji, Kabashima, Kenji, Furuyashiki, Tomoyuki, Murata, Takahiko, Segi-Nishida, Eri, Soontrapa, Kitipong, Matsuoka, Toshiyuki, Miyachi, Yoshiki, Narumiya, Shuh
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118516/
https://www.ncbi.nlm.nih.gov/pubmed/17967902
http://dx.doi.org/10.1084/jem.20070773
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author Nagamachi, Miyako
Sakata, Daiji
Kabashima, Kenji
Furuyashiki, Tomoyuki
Murata, Takahiko
Segi-Nishida, Eri
Soontrapa, Kitipong
Matsuoka, Toshiyuki
Miyachi, Yoshiki
Narumiya, Shuh
author_facet Nagamachi, Miyako
Sakata, Daiji
Kabashima, Kenji
Furuyashiki, Tomoyuki
Murata, Takahiko
Segi-Nishida, Eri
Soontrapa, Kitipong
Matsuoka, Toshiyuki
Miyachi, Yoshiki
Narumiya, Shuh
author_sort Nagamachi, Miyako
collection PubMed
description Prostaglandin E(2) (PGE(2)) exerts its actions via four subtypes of the PGE receptor, EP1–4. We show that mice deficient in EP1 exhibited significantly attenuated Th1 response in contact hypersensitivity induced by dinitrofluorobenzene (DNFB). This phenotype was recapitulated in wild-type mice by administration of an EP1-selective antagonist during the sensitization phase, and by adoptive transfer of T cells from sensitized EP1(−/−) mice. Conversely, an EP1-selective agonist facilitated Th1 differentiation of naive T cells in vitro. Finally, CD11c(+) cells containing the inducible form of PGE synthase increased in number in the draining lymph nodes after DNFB application. These results suggest that PGE(2) produced by dendritic cells in the lymph nodes acts on EP1 in naive T cells to promote Th1 differentiation.
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spelling pubmed-21185162008-05-26 Facilitation of Th1-mediated immune response by prostaglandin E receptor EP1 Nagamachi, Miyako Sakata, Daiji Kabashima, Kenji Furuyashiki, Tomoyuki Murata, Takahiko Segi-Nishida, Eri Soontrapa, Kitipong Matsuoka, Toshiyuki Miyachi, Yoshiki Narumiya, Shuh J Exp Med Articles Prostaglandin E(2) (PGE(2)) exerts its actions via four subtypes of the PGE receptor, EP1–4. We show that mice deficient in EP1 exhibited significantly attenuated Th1 response in contact hypersensitivity induced by dinitrofluorobenzene (DNFB). This phenotype was recapitulated in wild-type mice by administration of an EP1-selective antagonist during the sensitization phase, and by adoptive transfer of T cells from sensitized EP1(−/−) mice. Conversely, an EP1-selective agonist facilitated Th1 differentiation of naive T cells in vitro. Finally, CD11c(+) cells containing the inducible form of PGE synthase increased in number in the draining lymph nodes after DNFB application. These results suggest that PGE(2) produced by dendritic cells in the lymph nodes acts on EP1 in naive T cells to promote Th1 differentiation. The Rockefeller University Press 2007-11-26 /pmc/articles/PMC2118516/ /pubmed/17967902 http://dx.doi.org/10.1084/jem.20070773 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Nagamachi, Miyako
Sakata, Daiji
Kabashima, Kenji
Furuyashiki, Tomoyuki
Murata, Takahiko
Segi-Nishida, Eri
Soontrapa, Kitipong
Matsuoka, Toshiyuki
Miyachi, Yoshiki
Narumiya, Shuh
Facilitation of Th1-mediated immune response by prostaglandin E receptor EP1
title Facilitation of Th1-mediated immune response by prostaglandin E receptor EP1
title_full Facilitation of Th1-mediated immune response by prostaglandin E receptor EP1
title_fullStr Facilitation of Th1-mediated immune response by prostaglandin E receptor EP1
title_full_unstemmed Facilitation of Th1-mediated immune response by prostaglandin E receptor EP1
title_short Facilitation of Th1-mediated immune response by prostaglandin E receptor EP1
title_sort facilitation of th1-mediated immune response by prostaglandin e receptor ep1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118516/
https://www.ncbi.nlm.nih.gov/pubmed/17967902
http://dx.doi.org/10.1084/jem.20070773
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