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Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus
We defined the function of type I interferons (IFNs) in defense against reovirus strain type 1 Lang (T1L), which is a double-stranded RNA virus that infects Peyer's patches (PPs) after peroral inoculation of mice. T1L induced expression of mRNA for IFN-α, IFN-β, and Mx-1 in PPs and caused local...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118611/ https://www.ncbi.nlm.nih.gov/pubmed/17502662 http://dx.doi.org/10.1084/jem.20061587 |
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author | Johansson, Cecilia Wetzel, J. Denise He, JianPing Mikacenic, Carmen Dermody, Terence S. Kelsall, Brian L. |
author_facet | Johansson, Cecilia Wetzel, J. Denise He, JianPing Mikacenic, Carmen Dermody, Terence S. Kelsall, Brian L. |
author_sort | Johansson, Cecilia |
collection | PubMed |
description | We defined the function of type I interferons (IFNs) in defense against reovirus strain type 1 Lang (T1L), which is a double-stranded RNA virus that infects Peyer's patches (PPs) after peroral inoculation of mice. T1L induced expression of mRNA for IFN-α, IFN-β, and Mx-1 in PPs and caused localized intestinal infection that was cleared in 10 d. In contrast, T1L produced fatal systemic infection in IFNαR1 knockout (KO) mice with extensive cell loss in lymphoid tissues and necrosis of the intestinal mucosa. Studies of bone-marrow chimeric mice indicated an essential role for hematopoietic cells in IFN-dependent viral clearance. Dendritic cells (DCs), including conventional DCs (cDCs), were the major source of type I IFNs in PPs of reovirus-infected mice, whereas all cell types expressed the antiviral protein Mx-1. Neither NK cells nor signaling via Toll-like receptor 3 or MyD88 were essential for viral clearance. These data demonstrate a requirement for type I IFNs in the control of an intestinal viral infection and indicate that cDCs are a significant source of type I IFN production in vivo. Therefore, innate immunity in PPs is an essential component of host defense that limits systemic spread of pathogens that infect the intestinal mucosa. |
format | Text |
id | pubmed-2118611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21186112007-12-13 Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus Johansson, Cecilia Wetzel, J. Denise He, JianPing Mikacenic, Carmen Dermody, Terence S. Kelsall, Brian L. J Exp Med Articles We defined the function of type I interferons (IFNs) in defense against reovirus strain type 1 Lang (T1L), which is a double-stranded RNA virus that infects Peyer's patches (PPs) after peroral inoculation of mice. T1L induced expression of mRNA for IFN-α, IFN-β, and Mx-1 in PPs and caused localized intestinal infection that was cleared in 10 d. In contrast, T1L produced fatal systemic infection in IFNαR1 knockout (KO) mice with extensive cell loss in lymphoid tissues and necrosis of the intestinal mucosa. Studies of bone-marrow chimeric mice indicated an essential role for hematopoietic cells in IFN-dependent viral clearance. Dendritic cells (DCs), including conventional DCs (cDCs), were the major source of type I IFNs in PPs of reovirus-infected mice, whereas all cell types expressed the antiviral protein Mx-1. Neither NK cells nor signaling via Toll-like receptor 3 or MyD88 were essential for viral clearance. These data demonstrate a requirement for type I IFNs in the control of an intestinal viral infection and indicate that cDCs are a significant source of type I IFN production in vivo. Therefore, innate immunity in PPs is an essential component of host defense that limits systemic spread of pathogens that infect the intestinal mucosa. The Rockefeller University Press 2007-06-11 /pmc/articles/PMC2118611/ /pubmed/17502662 http://dx.doi.org/10.1084/jem.20061587 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Johansson, Cecilia Wetzel, J. Denise He, JianPing Mikacenic, Carmen Dermody, Terence S. Kelsall, Brian L. Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus |
title | Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus |
title_full | Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus |
title_fullStr | Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus |
title_full_unstemmed | Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus |
title_short | Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus |
title_sort | type i interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118611/ https://www.ncbi.nlm.nih.gov/pubmed/17502662 http://dx.doi.org/10.1084/jem.20061587 |
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