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Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus

We defined the function of type I interferons (IFNs) in defense against reovirus strain type 1 Lang (T1L), which is a double-stranded RNA virus that infects Peyer's patches (PPs) after peroral inoculation of mice. T1L induced expression of mRNA for IFN-α, IFN-β, and Mx-1 in PPs and caused local...

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Autores principales: Johansson, Cecilia, Wetzel, J. Denise, He, JianPing, Mikacenic, Carmen, Dermody, Terence S., Kelsall, Brian L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118611/
https://www.ncbi.nlm.nih.gov/pubmed/17502662
http://dx.doi.org/10.1084/jem.20061587
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author Johansson, Cecilia
Wetzel, J. Denise
He, JianPing
Mikacenic, Carmen
Dermody, Terence S.
Kelsall, Brian L.
author_facet Johansson, Cecilia
Wetzel, J. Denise
He, JianPing
Mikacenic, Carmen
Dermody, Terence S.
Kelsall, Brian L.
author_sort Johansson, Cecilia
collection PubMed
description We defined the function of type I interferons (IFNs) in defense against reovirus strain type 1 Lang (T1L), which is a double-stranded RNA virus that infects Peyer's patches (PPs) after peroral inoculation of mice. T1L induced expression of mRNA for IFN-α, IFN-β, and Mx-1 in PPs and caused localized intestinal infection that was cleared in 10 d. In contrast, T1L produced fatal systemic infection in IFNαR1 knockout (KO) mice with extensive cell loss in lymphoid tissues and necrosis of the intestinal mucosa. Studies of bone-marrow chimeric mice indicated an essential role for hematopoietic cells in IFN-dependent viral clearance. Dendritic cells (DCs), including conventional DCs (cDCs), were the major source of type I IFNs in PPs of reovirus-infected mice, whereas all cell types expressed the antiviral protein Mx-1. Neither NK cells nor signaling via Toll-like receptor 3 or MyD88 were essential for viral clearance. These data demonstrate a requirement for type I IFNs in the control of an intestinal viral infection and indicate that cDCs are a significant source of type I IFN production in vivo. Therefore, innate immunity in PPs is an essential component of host defense that limits systemic spread of pathogens that infect the intestinal mucosa.
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spelling pubmed-21186112007-12-13 Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus Johansson, Cecilia Wetzel, J. Denise He, JianPing Mikacenic, Carmen Dermody, Terence S. Kelsall, Brian L. J Exp Med Articles We defined the function of type I interferons (IFNs) in defense against reovirus strain type 1 Lang (T1L), which is a double-stranded RNA virus that infects Peyer's patches (PPs) after peroral inoculation of mice. T1L induced expression of mRNA for IFN-α, IFN-β, and Mx-1 in PPs and caused localized intestinal infection that was cleared in 10 d. In contrast, T1L produced fatal systemic infection in IFNαR1 knockout (KO) mice with extensive cell loss in lymphoid tissues and necrosis of the intestinal mucosa. Studies of bone-marrow chimeric mice indicated an essential role for hematopoietic cells in IFN-dependent viral clearance. Dendritic cells (DCs), including conventional DCs (cDCs), were the major source of type I IFNs in PPs of reovirus-infected mice, whereas all cell types expressed the antiviral protein Mx-1. Neither NK cells nor signaling via Toll-like receptor 3 or MyD88 were essential for viral clearance. These data demonstrate a requirement for type I IFNs in the control of an intestinal viral infection and indicate that cDCs are a significant source of type I IFN production in vivo. Therefore, innate immunity in PPs is an essential component of host defense that limits systemic spread of pathogens that infect the intestinal mucosa. The Rockefeller University Press 2007-06-11 /pmc/articles/PMC2118611/ /pubmed/17502662 http://dx.doi.org/10.1084/jem.20061587 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Johansson, Cecilia
Wetzel, J. Denise
He, JianPing
Mikacenic, Carmen
Dermody, Terence S.
Kelsall, Brian L.
Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus
title Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus
title_full Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus
title_fullStr Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus
title_full_unstemmed Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus
title_short Type I interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus
title_sort type i interferons produced by hematopoietic cells protect mice against lethal infection by mammalian reovirus
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118611/
https://www.ncbi.nlm.nih.gov/pubmed/17502662
http://dx.doi.org/10.1084/jem.20061587
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