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Thymus-grafted SCID mice show transient thymopoiesis and limited depletion of V beta 11+ T cells

To seek direct evidence for the notion that stem cells in the thymus need to be constantly replenished from the bone marrow (BM), fetal (day 15) thymuses from normal BALB/c mice were grafted into T and B cell- deficient C.B-17 SCID mice (both H-2d, I-E+). The thymus grafts in these mice showed norma...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1992
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119181/
https://www.ncbi.nlm.nih.gov/pubmed/1532413
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description To seek direct evidence for the notion that stem cells in the thymus need to be constantly replenished from the bone marrow (BM), fetal (day 15) thymuses from normal BALB/c mice were grafted into T and B cell- deficient C.B-17 SCID mice (both H-2d, I-E+). The thymus grafts in these mice showed normal thymopoiesis for the first 3 wk postgrafting but then developed sudden atrophy with near complete loss of CD4+8+ cells by 4-5 wk. Such atrophy was not seen when the thymus-grafted mice were cotransplanted with normal BM cells. The lymph nodes of SCID mice receiving thymus grafts alone contained mature T cells but virtually no B cells. This lack of B cells was associated with aberrant I-E- restricted V beta deletion: the depletion of V beta 3+ and V beta 5+ T cells was near complete, whereas V beta 11+ cells showed only marginal depletion.
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spelling pubmed-21191812008-04-16 Thymus-grafted SCID mice show transient thymopoiesis and limited depletion of V beta 11+ T cells J Exp Med Articles To seek direct evidence for the notion that stem cells in the thymus need to be constantly replenished from the bone marrow (BM), fetal (day 15) thymuses from normal BALB/c mice were grafted into T and B cell- deficient C.B-17 SCID mice (both H-2d, I-E+). The thymus grafts in these mice showed normal thymopoiesis for the first 3 wk postgrafting but then developed sudden atrophy with near complete loss of CD4+8+ cells by 4-5 wk. Such atrophy was not seen when the thymus-grafted mice were cotransplanted with normal BM cells. The lymph nodes of SCID mice receiving thymus grafts alone contained mature T cells but virtually no B cells. This lack of B cells was associated with aberrant I-E- restricted V beta deletion: the depletion of V beta 3+ and V beta 5+ T cells was near complete, whereas V beta 11+ cells showed only marginal depletion. The Rockefeller University Press 1992-04-01 /pmc/articles/PMC2119181/ /pubmed/1532413 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Thymus-grafted SCID mice show transient thymopoiesis and limited depletion of V beta 11+ T cells
title Thymus-grafted SCID mice show transient thymopoiesis and limited depletion of V beta 11+ T cells
title_full Thymus-grafted SCID mice show transient thymopoiesis and limited depletion of V beta 11+ T cells
title_fullStr Thymus-grafted SCID mice show transient thymopoiesis and limited depletion of V beta 11+ T cells
title_full_unstemmed Thymus-grafted SCID mice show transient thymopoiesis and limited depletion of V beta 11+ T cells
title_short Thymus-grafted SCID mice show transient thymopoiesis and limited depletion of V beta 11+ T cells
title_sort thymus-grafted scid mice show transient thymopoiesis and limited depletion of v beta 11+ t cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119181/
https://www.ncbi.nlm.nih.gov/pubmed/1532413