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Isolation of a matrix that binds medial Golgi enzymes
Rat liver Golgi stacks were extracted with Triton X-100 at neutral pH. After centrifugation the low speed pellet contained two medial-Golgi enzymes, N-acetylglucosaminyltransferase I and mannosidase II, but no enzymes or markers from other parts of the Golgi apparatus. Both were present in the same...
Formato: | Texto |
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Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1994
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119912/ https://www.ncbi.nlm.nih.gov/pubmed/8106542 |