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Isolation and characterization of nuclear envelopes from the yeast Saccharomyces
We have developed a large scale enrichment procedure to prepare yeast nuclear envelopes (NEs). These NEs can be stripped of peripheral proteins to produce a heparin-extracted NE (H-NE) fraction highly enriched in integral membrane proteins. Extraction of H-NEs with detergents revealed previously unc...
Formato: | Texto |
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Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1995
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120596/ https://www.ncbi.nlm.nih.gov/pubmed/7559775 |