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THE RÔLE OF CARBOHYDRATES IN BIOLOGICAL OXIDATIONS AND REDUCTIONS. EXPERIMENTS WITH PNEUMOCOCCUS

The reducing power of plain broth cultures of Pneumococcus is largely dependent upon the presence in the medium at the time when the reduction test is performed of certain metabolites. The washed cells of Pneumococcus are able to reduce the various indicators of oxidation-reduction potentials in the...

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Autor principal: Dubos, René
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1929
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2131618/
https://www.ncbi.nlm.nih.gov/pubmed/19869610
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author Dubos, René
author_facet Dubos, René
author_sort Dubos, René
collection PubMed
description The reducing power of plain broth cultures of Pneumococcus is largely dependent upon the presence in the medium at the time when the reduction test is performed of certain metabolites. The washed cells of Pneumococcus are able to reduce the various indicators of oxidation-reduction potentials in the presence of glucose. The relative velocity of reduction of these indicators is determined by the number of cells used in the test, the concentration of the dyes, and their position in the oxidation reduction scale. Oxidized thiol compounds (glutathione, cystine, oxidized thioglycollic acid) are likewise rapidly reduced by glucose in the presence of washed cells of Pneumococcus. This Pneumococcus-glucose system is able to form peroxide under aerobic conditions. Those substances which form peroxide in the presence of Pneumococcus cells are also the ones which Cole found to be active in changing hemoglobin into methemoglobin under the same conditions. The power of washed cells of Pneumococcus to reduce methylene blue in the presence of glucose is dependent on at least 2 constituents: one which can be readily removed from the cell by washing. Sugar-free meat infusion will function instead of it. The other is inactivated more slowly by the process of washing and is destroyed by 10 minutes heating at 55°C. The interreaction between the glucose and the cell seems to result in a fundamental reaction in which one molecule of glucose becomes able to reduce rapidly one molecule of methylene blue. The existence of side-reactions often obscures this ratio. The significance of these observations is considered in relation to the nature and mechanism of the "activation" of metabolites, the preparation of synthetic media, the phenomena of growth, and the meaning of the expression "reducing power of a bacterial culture."
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spelling pubmed-21316182008-04-18 THE RÔLE OF CARBOHYDRATES IN BIOLOGICAL OXIDATIONS AND REDUCTIONS. EXPERIMENTS WITH PNEUMOCOCCUS Dubos, René J Exp Med Article The reducing power of plain broth cultures of Pneumococcus is largely dependent upon the presence in the medium at the time when the reduction test is performed of certain metabolites. The washed cells of Pneumococcus are able to reduce the various indicators of oxidation-reduction potentials in the presence of glucose. The relative velocity of reduction of these indicators is determined by the number of cells used in the test, the concentration of the dyes, and their position in the oxidation reduction scale. Oxidized thiol compounds (glutathione, cystine, oxidized thioglycollic acid) are likewise rapidly reduced by glucose in the presence of washed cells of Pneumococcus. This Pneumococcus-glucose system is able to form peroxide under aerobic conditions. Those substances which form peroxide in the presence of Pneumococcus cells are also the ones which Cole found to be active in changing hemoglobin into methemoglobin under the same conditions. The power of washed cells of Pneumococcus to reduce methylene blue in the presence of glucose is dependent on at least 2 constituents: one which can be readily removed from the cell by washing. Sugar-free meat infusion will function instead of it. The other is inactivated more slowly by the process of washing and is destroyed by 10 minutes heating at 55°C. The interreaction between the glucose and the cell seems to result in a fundamental reaction in which one molecule of glucose becomes able to reduce rapidly one molecule of methylene blue. The existence of side-reactions often obscures this ratio. The significance of these observations is considered in relation to the nature and mechanism of the "activation" of metabolites, the preparation of synthetic media, the phenomena of growth, and the meaning of the expression "reducing power of a bacterial culture." The Rockefeller University Press 1929-07-31 /pmc/articles/PMC2131618/ /pubmed/19869610 Text en Copyright © Copyright, 1929, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Dubos, René
THE RÔLE OF CARBOHYDRATES IN BIOLOGICAL OXIDATIONS AND REDUCTIONS. EXPERIMENTS WITH PNEUMOCOCCUS
title THE RÔLE OF CARBOHYDRATES IN BIOLOGICAL OXIDATIONS AND REDUCTIONS. EXPERIMENTS WITH PNEUMOCOCCUS
title_full THE RÔLE OF CARBOHYDRATES IN BIOLOGICAL OXIDATIONS AND REDUCTIONS. EXPERIMENTS WITH PNEUMOCOCCUS
title_fullStr THE RÔLE OF CARBOHYDRATES IN BIOLOGICAL OXIDATIONS AND REDUCTIONS. EXPERIMENTS WITH PNEUMOCOCCUS
title_full_unstemmed THE RÔLE OF CARBOHYDRATES IN BIOLOGICAL OXIDATIONS AND REDUCTIONS. EXPERIMENTS WITH PNEUMOCOCCUS
title_short THE RÔLE OF CARBOHYDRATES IN BIOLOGICAL OXIDATIONS AND REDUCTIONS. EXPERIMENTS WITH PNEUMOCOCCUS
title_sort rôle of carbohydrates in biological oxidations and reductions. experiments with pneumococcus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2131618/
https://www.ncbi.nlm.nih.gov/pubmed/19869610
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