A Novel Fission Yeast Gene, tht1 (+), Is Required for the Fusion of Nuclear Envelopes during Karyogamy

We have isolated a fission yeast karyogamy mutant, tht1, in which nuclear congression and the association of two spindle pole bodies occurs but the subsequent fusion of nuclear envelopes is blocked. The tht1 mutation does not prevent meiosis, so cells execute meiosis with two unfused nuclei, leading...

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Autores principales: Tange, Yoshie, Horio, Tetsuya, Shimanuki, Mizuki, Ding, Da-Qiao, Hiraoka, Yasushi, Niwa, Osami
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132580/
https://www.ncbi.nlm.nih.gov/pubmed/9442101
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author Tange, Yoshie
Horio, Tetsuya
Shimanuki, Mizuki
Ding, Da-Qiao
Hiraoka, Yasushi
Niwa, Osami
author_facet Tange, Yoshie
Horio, Tetsuya
Shimanuki, Mizuki
Ding, Da-Qiao
Hiraoka, Yasushi
Niwa, Osami
author_sort Tange, Yoshie
collection PubMed
description We have isolated a fission yeast karyogamy mutant, tht1, in which nuclear congression and the association of two spindle pole bodies occurs but the subsequent fusion of nuclear envelopes is blocked. The tht1 mutation does not prevent meiosis, so cells execute meiosis with two unfused nuclei, leading to the production of aberrant asci. The tht1 (+) gene was cloned and sequenced. Predicted amino acid sequence has no significant homology to previously known proteins but strongly suggests that it is a type I membrane protein. The tht1 (+) gene is dispensable for vegetative growth and expressed only in conjugating cells. Tht1p is a glycoprotein susceptible to endoglycosilase H digestion. Site- directed mutagenesis showed that the N-glycosylation site, as well as the COOH-terminal region of Tht1p, is essential for its function. A protease protection assay indicated that the COOH terminus is cytoplasmic. Immunocytological analysis using a HA-tagged Tht1p suggested that the protein is localized in nuclear envelopes and in the ER during karyogamy and that its levels are reduced in cells containing fused nuclei.
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spelling pubmed-21325802008-05-01 A Novel Fission Yeast Gene, tht1 (+), Is Required for the Fusion of Nuclear Envelopes during Karyogamy Tange, Yoshie Horio, Tetsuya Shimanuki, Mizuki Ding, Da-Qiao Hiraoka, Yasushi Niwa, Osami J Cell Biol Article We have isolated a fission yeast karyogamy mutant, tht1, in which nuclear congression and the association of two spindle pole bodies occurs but the subsequent fusion of nuclear envelopes is blocked. The tht1 mutation does not prevent meiosis, so cells execute meiosis with two unfused nuclei, leading to the production of aberrant asci. The tht1 (+) gene was cloned and sequenced. Predicted amino acid sequence has no significant homology to previously known proteins but strongly suggests that it is a type I membrane protein. The tht1 (+) gene is dispensable for vegetative growth and expressed only in conjugating cells. Tht1p is a glycoprotein susceptible to endoglycosilase H digestion. Site- directed mutagenesis showed that the N-glycosylation site, as well as the COOH-terminal region of Tht1p, is essential for its function. A protease protection assay indicated that the COOH terminus is cytoplasmic. Immunocytological analysis using a HA-tagged Tht1p suggested that the protein is localized in nuclear envelopes and in the ER during karyogamy and that its levels are reduced in cells containing fused nuclei. The Rockefeller University Press 1998-01-26 /pmc/articles/PMC2132580/ /pubmed/9442101 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Tange, Yoshie
Horio, Tetsuya
Shimanuki, Mizuki
Ding, Da-Qiao
Hiraoka, Yasushi
Niwa, Osami
A Novel Fission Yeast Gene, tht1 (+), Is Required for the Fusion of Nuclear Envelopes during Karyogamy
title A Novel Fission Yeast Gene, tht1 (+), Is Required for the Fusion of Nuclear Envelopes during Karyogamy
title_full A Novel Fission Yeast Gene, tht1 (+), Is Required for the Fusion of Nuclear Envelopes during Karyogamy
title_fullStr A Novel Fission Yeast Gene, tht1 (+), Is Required for the Fusion of Nuclear Envelopes during Karyogamy
title_full_unstemmed A Novel Fission Yeast Gene, tht1 (+), Is Required for the Fusion of Nuclear Envelopes during Karyogamy
title_short A Novel Fission Yeast Gene, tht1 (+), Is Required for the Fusion of Nuclear Envelopes during Karyogamy
title_sort novel fission yeast gene, tht1 (+), is required for the fusion of nuclear envelopes during karyogamy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132580/
https://www.ncbi.nlm.nih.gov/pubmed/9442101
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