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Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis

Protein kinase C β(II) (PKC β(II)) has been implicated in proliferation of the intestinal epithelium. To investigate PKC β(II) function in vivo, we generated transgenic mice that overexpress PKC β(II) in the intestinal epithelium. Transgenic PKC β(II) mice exhibit hyperproliferation of the colonic e...

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Autores principales: Murray, Nicole R., Davidson, Laurie A., Chapkin, Robert S., Clay Gustafson, W., Schattenberg, Diane G., Fields, Alan P.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133187/
https://www.ncbi.nlm.nih.gov/pubmed/10330400
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author Murray, Nicole R.
Davidson, Laurie A.
Chapkin, Robert S.
Clay Gustafson, W.
Schattenberg, Diane G.
Fields, Alan P.
author_facet Murray, Nicole R.
Davidson, Laurie A.
Chapkin, Robert S.
Clay Gustafson, W.
Schattenberg, Diane G.
Fields, Alan P.
author_sort Murray, Nicole R.
collection PubMed
description Protein kinase C β(II) (PKC β(II)) has been implicated in proliferation of the intestinal epithelium. To investigate PKC β(II) function in vivo, we generated transgenic mice that overexpress PKC β(II) in the intestinal epithelium. Transgenic PKC β(II) mice exhibit hyperproliferation of the colonic epithelium and an increased susceptibility to azoxymethane-induced aberrant crypt foci, preneoplastic lesions in the colon. Furthermore, transgenic PKC β(II) mice exhibit elevated colonic β-catenin levels and decreased glycogen synthase kinase 3β activity, indicating that PKC β(II) stimulates the Wnt/adenomatous polyposis coli (APC)/β-catenin proliferative signaling pathway in vivo. These data demonstrate a direct role for PKC β(II) in colonic epithelial cell proliferation and colon carcinogenesis, possibly through activation of the APC/β-catenin signaling pathway.
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spelling pubmed-21331872008-05-01 Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis Murray, Nicole R. Davidson, Laurie A. Chapkin, Robert S. Clay Gustafson, W. Schattenberg, Diane G. Fields, Alan P. J Cell Biol Regular Articles Protein kinase C β(II) (PKC β(II)) has been implicated in proliferation of the intestinal epithelium. To investigate PKC β(II) function in vivo, we generated transgenic mice that overexpress PKC β(II) in the intestinal epithelium. Transgenic PKC β(II) mice exhibit hyperproliferation of the colonic epithelium and an increased susceptibility to azoxymethane-induced aberrant crypt foci, preneoplastic lesions in the colon. Furthermore, transgenic PKC β(II) mice exhibit elevated colonic β-catenin levels and decreased glycogen synthase kinase 3β activity, indicating that PKC β(II) stimulates the Wnt/adenomatous polyposis coli (APC)/β-catenin proliferative signaling pathway in vivo. These data demonstrate a direct role for PKC β(II) in colonic epithelial cell proliferation and colon carcinogenesis, possibly through activation of the APC/β-catenin signaling pathway. The Rockefeller University Press 1999-05-17 /pmc/articles/PMC2133187/ /pubmed/10330400 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Regular Articles
Murray, Nicole R.
Davidson, Laurie A.
Chapkin, Robert S.
Clay Gustafson, W.
Schattenberg, Diane G.
Fields, Alan P.
Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis
title Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis
title_full Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis
title_fullStr Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis
title_full_unstemmed Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis
title_short Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis
title_sort overexpression of protein kinase c β(ii) induces colonic hyperproliferation and increased sensitivity to colon carcinogenesis
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133187/
https://www.ncbi.nlm.nih.gov/pubmed/10330400
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