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Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis
Protein kinase C β(II) (PKC β(II)) has been implicated in proliferation of the intestinal epithelium. To investigate PKC β(II) function in vivo, we generated transgenic mice that overexpress PKC β(II) in the intestinal epithelium. Transgenic PKC β(II) mice exhibit hyperproliferation of the colonic e...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133187/ https://www.ncbi.nlm.nih.gov/pubmed/10330400 |
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author | Murray, Nicole R. Davidson, Laurie A. Chapkin, Robert S. Clay Gustafson, W. Schattenberg, Diane G. Fields, Alan P. |
author_facet | Murray, Nicole R. Davidson, Laurie A. Chapkin, Robert S. Clay Gustafson, W. Schattenberg, Diane G. Fields, Alan P. |
author_sort | Murray, Nicole R. |
collection | PubMed |
description | Protein kinase C β(II) (PKC β(II)) has been implicated in proliferation of the intestinal epithelium. To investigate PKC β(II) function in vivo, we generated transgenic mice that overexpress PKC β(II) in the intestinal epithelium. Transgenic PKC β(II) mice exhibit hyperproliferation of the colonic epithelium and an increased susceptibility to azoxymethane-induced aberrant crypt foci, preneoplastic lesions in the colon. Furthermore, transgenic PKC β(II) mice exhibit elevated colonic β-catenin levels and decreased glycogen synthase kinase 3β activity, indicating that PKC β(II) stimulates the Wnt/adenomatous polyposis coli (APC)/β-catenin proliferative signaling pathway in vivo. These data demonstrate a direct role for PKC β(II) in colonic epithelial cell proliferation and colon carcinogenesis, possibly through activation of the APC/β-catenin signaling pathway. |
format | Text |
id | pubmed-2133187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21331872008-05-01 Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis Murray, Nicole R. Davidson, Laurie A. Chapkin, Robert S. Clay Gustafson, W. Schattenberg, Diane G. Fields, Alan P. J Cell Biol Regular Articles Protein kinase C β(II) (PKC β(II)) has been implicated in proliferation of the intestinal epithelium. To investigate PKC β(II) function in vivo, we generated transgenic mice that overexpress PKC β(II) in the intestinal epithelium. Transgenic PKC β(II) mice exhibit hyperproliferation of the colonic epithelium and an increased susceptibility to azoxymethane-induced aberrant crypt foci, preneoplastic lesions in the colon. Furthermore, transgenic PKC β(II) mice exhibit elevated colonic β-catenin levels and decreased glycogen synthase kinase 3β activity, indicating that PKC β(II) stimulates the Wnt/adenomatous polyposis coli (APC)/β-catenin proliferative signaling pathway in vivo. These data demonstrate a direct role for PKC β(II) in colonic epithelial cell proliferation and colon carcinogenesis, possibly through activation of the APC/β-catenin signaling pathway. The Rockefeller University Press 1999-05-17 /pmc/articles/PMC2133187/ /pubmed/10330400 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Regular Articles Murray, Nicole R. Davidson, Laurie A. Chapkin, Robert S. Clay Gustafson, W. Schattenberg, Diane G. Fields, Alan P. Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis |
title | Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis |
title_full | Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis |
title_fullStr | Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis |
title_full_unstemmed | Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis |
title_short | Overexpression of Protein Kinase C β(II) Induces Colonic Hyperproliferation and Increased Sensitivity to Colon Carcinogenesis |
title_sort | overexpression of protein kinase c β(ii) induces colonic hyperproliferation and increased sensitivity to colon carcinogenesis |
topic | Regular Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133187/ https://www.ncbi.nlm.nih.gov/pubmed/10330400 |
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