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A method for the construction of equalized directional cDNA libraries from hydrolyzed total RNA
BACKGROUND: The transcribed sequences of a cell, the transcriptome, represent the trans-acting fraction of the genetic information, yet eukaryotic cDNA libraries are typically made from only the poly-adenylated fraction. The non-coding or translated but non-polyadenylated RNAs are therefore not repr...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2134933/ https://www.ncbi.nlm.nih.gov/pubmed/17925018 http://dx.doi.org/10.1186/1471-2164-8-363 |