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A Dual-Specificity Phosphatase Cdc25B Is an Unstable Protein and Triggers p34(cdc2)/Cyclin B Activation in Hamster BHK21 Cells Arrested with Hydroxyurea
By incubating at 30°C in the presence of an energy source, p34(cdc2)/cyclin B was activated in the extract prepared from a temperature-sensitive mutant, tsBN2, which prematurely enters mitosis at 40°C, the nonpermissive temperature (Nishimoto, T., E. Eilen, and C. Basilico. 1978. Cell. 15:475–483),...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1997
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136770/ https://www.ncbi.nlm.nih.gov/pubmed/9281587 |