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GROUP A STREPTOCOCCAL BACTERIOCIN : PRODUCTION, PURIFICATION, AND MODE OF ACTION

A bacteriocin, streptocin A, was isolated from the supernatant fluid of tryptic soy broth cultures of Group A streptococcus strain FF-22. Evidence was obtained which supports the view that the failure to recover active streptocin A after growth of the producer strain in certain fluid media is due to...

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Autores principales: Tagg, John R., Dajani, Adnan S., Wannamaker, Lewis W., Gray, Ernest D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139428/
https://www.ncbi.nlm.nih.gov/pubmed/4744922
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author Tagg, John R.
Dajani, Adnan S.
Wannamaker, Lewis W.
Gray, Ernest D.
author_facet Tagg, John R.
Dajani, Adnan S.
Wannamaker, Lewis W.
Gray, Ernest D.
author_sort Tagg, John R.
collection PubMed
description A bacteriocin, streptocin A, was isolated from the supernatant fluid of tryptic soy broth cultures of Group A streptococcus strain FF-22. Evidence was obtained which supports the view that the failure to recover active streptocin A after growth of the producer strain in certain fluid media is due to the inactivation of the bacteriocin by concomitantly synthesized streptococcal proteinase. The bacteriocin was purified 139-fold and the active product appeared to be of uniform size, having a molecular weight of approximately 8,000. Streptocin A was bactericidal, but not lytic, for a susceptible Group A streptococcus and the lethal effect was markedly temperature dependent. The bacteriocin inhibited the synthesis of DNA, RNA, and protein, and also prevented the uptake and incorporation of glucose by the sensitive cells. Degradation of RNA occurred, but appeared to be less than that produced by a staphylococcal bacteriocin. This effect may be due to differences in the killing potency of the two bacteriocins in preparations having similar inhibitory activity when measured by lawn culture assays.
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spelling pubmed-21394282008-04-17 GROUP A STREPTOCOCCAL BACTERIOCIN : PRODUCTION, PURIFICATION, AND MODE OF ACTION Tagg, John R. Dajani, Adnan S. Wannamaker, Lewis W. Gray, Ernest D. J Exp Med Article A bacteriocin, streptocin A, was isolated from the supernatant fluid of tryptic soy broth cultures of Group A streptococcus strain FF-22. Evidence was obtained which supports the view that the failure to recover active streptocin A after growth of the producer strain in certain fluid media is due to the inactivation of the bacteriocin by concomitantly synthesized streptococcal proteinase. The bacteriocin was purified 139-fold and the active product appeared to be of uniform size, having a molecular weight of approximately 8,000. Streptocin A was bactericidal, but not lytic, for a susceptible Group A streptococcus and the lethal effect was markedly temperature dependent. The bacteriocin inhibited the synthesis of DNA, RNA, and protein, and also prevented the uptake and incorporation of glucose by the sensitive cells. Degradation of RNA occurred, but appeared to be less than that produced by a staphylococcal bacteriocin. This effect may be due to differences in the killing potency of the two bacteriocins in preparations having similar inhibitory activity when measured by lawn culture assays. The Rockefeller University Press 1973-10-31 /pmc/articles/PMC2139428/ /pubmed/4744922 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Tagg, John R.
Dajani, Adnan S.
Wannamaker, Lewis W.
Gray, Ernest D.
GROUP A STREPTOCOCCAL BACTERIOCIN : PRODUCTION, PURIFICATION, AND MODE OF ACTION
title GROUP A STREPTOCOCCAL BACTERIOCIN : PRODUCTION, PURIFICATION, AND MODE OF ACTION
title_full GROUP A STREPTOCOCCAL BACTERIOCIN : PRODUCTION, PURIFICATION, AND MODE OF ACTION
title_fullStr GROUP A STREPTOCOCCAL BACTERIOCIN : PRODUCTION, PURIFICATION, AND MODE OF ACTION
title_full_unstemmed GROUP A STREPTOCOCCAL BACTERIOCIN : PRODUCTION, PURIFICATION, AND MODE OF ACTION
title_short GROUP A STREPTOCOCCAL BACTERIOCIN : PRODUCTION, PURIFICATION, AND MODE OF ACTION
title_sort group a streptococcal bacteriocin : production, purification, and mode of action
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139428/
https://www.ncbi.nlm.nih.gov/pubmed/4744922
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