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SEPARATION OF HUMAN LYMPHOID CELLS INTO G(1), S, AND G(2) CELL CYCLE POPULATIONS BY USE OF A VELOCITY SEDIMENTATION TECHNIQUE
Human lymphoid tissue culture cells can be separated according to cell size and corresponding cell cycle phase with a velocity sedimentation centrifugation method employing a continuous 5–20% wt/wt Ficoll gradient. A 7-fold increase in streaming limit was achieved by placing a buffer zone of isosmol...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1973
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139489/ https://www.ncbi.nlm.nih.gov/pubmed/4568300 |