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ISOLATION OF ANTIGEN-BINDING CELLS FROM UNPRIMED MICE : Demonstration of Antibody-Forming Cell Precursor Activity and Correlation between Precursor and Secreted Antibody Avidities

Cells binding DNP groups conjugated to fluoresceinated mouse gamma globulin ((F)DNP-MGG) were isolated from spleens of unprimed mice using a fluorescence-activated cell sorter (FACS). The isolated cells were specifically enriched at least 100-fold for anti-DNP precursor activity in an adoptive trans...

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Detalles Bibliográficos
Autores principales: Julius, Michael H., Herzenberg, Leonard A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139630/
https://www.ncbi.nlm.nih.gov/pubmed/4139227