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The dermis contains langerin(+) dendritic cells that develop and function independently of epidermal Langerhans cells
Langerhans cells (LCs) constitute a subset of dendritic cells (DCs) that express the lectin langerin and that reside in their immature state in epidermis. Paradoxically, in mice permitting diphtheria toxin (DT)–mediated ablation of LCs, epidermal LCs reappeared with kinetics that lagged behind that...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150992/ https://www.ncbi.nlm.nih.gov/pubmed/18086861 http://dx.doi.org/10.1084/jem.20071724 |
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author | Poulin, Lionel Franz Henri, Sandrine de Bovis, Béatrice Devilard, Elisabeth Kissenpfennig, Adrien Malissen, Bernard |
author_facet | Poulin, Lionel Franz Henri, Sandrine de Bovis, Béatrice Devilard, Elisabeth Kissenpfennig, Adrien Malissen, Bernard |
author_sort | Poulin, Lionel Franz |
collection | PubMed |
description | Langerhans cells (LCs) constitute a subset of dendritic cells (DCs) that express the lectin langerin and that reside in their immature state in epidermis. Paradoxically, in mice permitting diphtheria toxin (DT)–mediated ablation of LCs, epidermal LCs reappeared with kinetics that lagged behind that of their putative progeny found in lymph nodes (LNs). Using bone marrow (BM) chimeras, we showed that a major fraction of the langerin(+), skin-derived DCs found in LNs originates from a developmental pathway that is independent from that of epidermal LCs. This pathway, the existence of which was unexpected, originates in the dermis and gives rise to langerin(+) dermal DCs (DDCs) that should not be confused with epidermal LCs en route to LNs. It explains that after DT treatment, some langerin(+), skin-derived DCs reappear in LNs long before LC-derived DCs. Using CD45 expression and BrdU-labeling kinetics, both LCs and langerin(+) DDCs were found to coexist in wild-type mice. Moreover, DT-mediated ablation of epidermal LCs opened otherwise filled niches and permitted repopulation of adult noninflammatory epidermis with BM-derived LCs. Our results stress that the langerin(+) DC network is more complex than originally thought and have implications for the development of transcutaneous vaccines and the improvement of humanized mouse models. |
format | Text |
id | pubmed-2150992 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21509922008-06-24 The dermis contains langerin(+) dendritic cells that develop and function independently of epidermal Langerhans cells Poulin, Lionel Franz Henri, Sandrine de Bovis, Béatrice Devilard, Elisabeth Kissenpfennig, Adrien Malissen, Bernard J Exp Med Articles Langerhans cells (LCs) constitute a subset of dendritic cells (DCs) that express the lectin langerin and that reside in their immature state in epidermis. Paradoxically, in mice permitting diphtheria toxin (DT)–mediated ablation of LCs, epidermal LCs reappeared with kinetics that lagged behind that of their putative progeny found in lymph nodes (LNs). Using bone marrow (BM) chimeras, we showed that a major fraction of the langerin(+), skin-derived DCs found in LNs originates from a developmental pathway that is independent from that of epidermal LCs. This pathway, the existence of which was unexpected, originates in the dermis and gives rise to langerin(+) dermal DCs (DDCs) that should not be confused with epidermal LCs en route to LNs. It explains that after DT treatment, some langerin(+), skin-derived DCs reappear in LNs long before LC-derived DCs. Using CD45 expression and BrdU-labeling kinetics, both LCs and langerin(+) DDCs were found to coexist in wild-type mice. Moreover, DT-mediated ablation of epidermal LCs opened otherwise filled niches and permitted repopulation of adult noninflammatory epidermis with BM-derived LCs. Our results stress that the langerin(+) DC network is more complex than originally thought and have implications for the development of transcutaneous vaccines and the improvement of humanized mouse models. The Rockefeller University Press 2007-12-24 /pmc/articles/PMC2150992/ /pubmed/18086861 http://dx.doi.org/10.1084/jem.20071724 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Poulin, Lionel Franz Henri, Sandrine de Bovis, Béatrice Devilard, Elisabeth Kissenpfennig, Adrien Malissen, Bernard The dermis contains langerin(+) dendritic cells that develop and function independently of epidermal Langerhans cells |
title | The dermis contains langerin(+) dendritic cells that develop and function independently of epidermal Langerhans cells |
title_full | The dermis contains langerin(+) dendritic cells that develop and function independently of epidermal Langerhans cells |
title_fullStr | The dermis contains langerin(+) dendritic cells that develop and function independently of epidermal Langerhans cells |
title_full_unstemmed | The dermis contains langerin(+) dendritic cells that develop and function independently of epidermal Langerhans cells |
title_short | The dermis contains langerin(+) dendritic cells that develop and function independently of epidermal Langerhans cells |
title_sort | dermis contains langerin(+) dendritic cells that develop and function independently of epidermal langerhans cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150992/ https://www.ncbi.nlm.nih.gov/pubmed/18086861 http://dx.doi.org/10.1084/jem.20071724 |
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