Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR

BACKGROUND: In functional genomics, transcript measurement is of fundamental importance. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays are the most popular technology and depend on the initial molecular step, the reverse transcription (RT). This study provides a compl...

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Detalles Bibliográficos
Autores principales: Levesque-Sergerie, Jean-Philippe, Duquette, Mathieu, Thibault, Catherine, Delbecchi, Louis, Bissonnette, Nathalie
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151766/
https://www.ncbi.nlm.nih.gov/pubmed/17953766
http://dx.doi.org/10.1186/1471-2199-8-93

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151766/
https://www.ncbi.nlm.nih.gov/pubmed/17953766
http://dx.doi.org/10.1186/1471-2199-8-93

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