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Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR
BACKGROUND: In functional genomics, transcript measurement is of fundamental importance. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays are the most popular technology and depend on the initial molecular step, the reverse transcription (RT). This study provides a compl...
Autores principales: | Levesque-Sergerie, Jean-Philippe, Duquette, Mathieu, Thibault, Catherine, Delbecchi, Louis, Bissonnette, Nathalie |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151766/ https://www.ncbi.nlm.nih.gov/pubmed/17953766 http://dx.doi.org/10.1186/1471-2199-8-93 |
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