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Evaluation of reference genes for real-time RT-PCR expression studies in the plant pathogen Pectobacterium atrosepticum

BACKGROUND: Real-time RT-PCR has become a powerful technique to monitor low-abundance mRNA expression and is a useful tool when examining bacterial gene expression inside infected host tissues. However, correct evaluation of data requires accurate and reliable normalisation against internal standard...

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Detalles Bibliográficos
Autores principales: Takle, Gunnhild W, Toth, Ian K, Brurberg, May B
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151947/
https://www.ncbi.nlm.nih.gov/pubmed/17888160
http://dx.doi.org/10.1186/1471-2229-7-50